Tabriz University of Medical Sciences Medical Journal of Tabriz University of Medical Sciences 2783-2031 39 3 2017 08 06 Study of active secondary metabolites of Streptomyces levis isolated from north west soils of Iran and their in vitro and in vivo antibacterial effects 39 46 EN Ali Reza Dehnad Rahib Abushov Javad Hamedi Mohammad Reza Nahaei Haedeh Mobaiyen Ali Zendehdel Journal Article 2017 07 09 Background: Streptomyces levis is a member of Actinomycetes family. Chemical compounds extracted from this bacterium have been used as antibiotics, anti-tumoral, anti-bacterial, anti-fungal, antiviral, anticancer factors. The purpose of this study‚ was to investigate different metabolites of Streptomyces strains isolated from north west soils, of Iran and antimicrobial effects of them. Methods: The soil samples were taken from north west of Iran in 2010. The isolated bacteria were purified and identified by conventional methods. The metabolites of this bacterium were extracted by 7 different solvents (diethyl ether, dichloromethane, hexane, ethyl acetate, chloroform, methanol and water). The antimicrobial effects of metabolites were tested by disk agar diffusion method on Gram-positive and Gram- negative bacteria. The metabolites with antibacterial effects were analyzed by gas chromatography-mass spectrometry (GC-MS). Results: Metabolites produced from diethyl ether solvent affected Gram- positive and Gram- negative strains and 15 different compounds identified in the active metabolite of the bacterium. The main ingredients were: Docosanoic acid, methyl ester (11.578%), 9-12-octadecadienoic acid (2.961%), 5-Tetracosenois acid, methyl ester (8.389%), Bis (2-ethylhexyl) phthalate (2.153%), and D-alpha-Tocopherol (0.959%). Conclusion: Results of this study showed that docosanoic acid, methyl ester (11.578%) and 5-tetracosenois acid methyl ester (8.389%), were the main metabolites of Streptomyces levis isolated from north west soils of Iran. Antimicrobial effects of extracts could be due to the presence of 1, 2-benzene dicarboxylic acid diisooctyl ester compounds and bis (2-ethylhexyl) phthalate in the metabolite.