Tabriz University of Medical Sciences Medical Journal of Tabriz University of Medical Sciences 2783-2031 42 2 2020 06 10 The response of BAX, BCL-2 ratio to vitamin D consumption with concurrent training in rat males 194 199 10.34172/mj.2020.036 EN samad safarzadeh gargari https://orcid.org/0000-0001-5972-1441 hassan matin homaei https://orcid.org/0000-0001-9340-1759 mohamad ali azarbayjani https://orcid.org/0000-0001-5333-9639 Journal Article 10.34172/mj.2020.036 2018 05 27 Background: Previous studies has been indicated that vitamin D regulate apoptosis of myocardial, and improve cardiac performance But this mechanism has been remain unclear. The aim of this study was the response of BAX, BCL-2 ratio to vitamin D consumption with concurrent training in rat males. Methods: 50 male’s rats were randomly assigned to 5 groups with 10 rats in which group. Groups include of: 1-control Group (C), 2- vitamin D, group (D), 3-exercise group (E), 4-exercise+vitamin D group (ED), and 5- d methyl sulfoxide + salin group (M). Exercise groups have been ran on treadmill for 8 weeks in every days with specific speed and incline. 24 hr after last exercise and in anesthetic state all rats have been knockouted to determine bax and bcl2 proteins ratio. For measuring the BAX and BCL-2 proteins were used by ELISA technic. Results: The amount of protein in E+D group was more decreased in compared to other groups but no significant difference has been observed in between groups (p=0.138). Also there was little altered in BCL-2 and BAX/BCL-2 ratio in E and D groups compared to control and placebo groups (P>0/05). Conclusion: The eight weeks concurrent training with vitamin D supplementation had no effect on BAX/BCL-2 protein ratio in rats, and to some extent can prevent the myocardial apoptosis process via Bax/Bcl-2 ratio regulating.