Tabriz University of Medical Sciences Medical Journal of Tabriz University of Medical Sciences 2783-2031 30 1 2008 03 22 Evaluation of Effect of Aminoglycosides(Gentamicin, Neomycin,Streptomycin) and Fluoroquinolones(Ofloxacin )antibiotics on Testis Apoptosis by TUNEL assay in Rat. 43 49 EN KHAKI ARASH GHAFARI M NOURI M KHAKI A BAZI P IBRAHIMNEJAD MA Journal Article 2010 01 24 Background and objectives: Gentamicin, neomycin, streptomycin and ofloxacin are synthetic antibacterial agents belonging to the family of aminoglycoside and fluoroquinolone antibiotics with a very broad spectrum against microbial pathogens, especially gram-negatives and urinary tract infections which in nowadays have good effect in diseases treatment world-wide. The aim of this study was to see the effects of this drugs inducement, on testis apoptosis by Tunel assay in rat. Materials and Methods: fifty male Wistar rat were selected and randomly divided into five groups; one control (n=10) and four test (n=10) each. The test groups was received 5mg/kg (IP) gentamicin,50mg/kg(IP) neomycin, 40mg/kg (IP) streptomycin, and72mg/kg (IP) ofloxacin daily for fourteenth day; however the control group just received vehicle(IP). In fourteenth day the testis tissue of rat in both groups were removed and prepared for light microscopy, and the Terminal Uridine Nick End Labeling (TUNEL) technique was used to identify apoptosis. Results: The rate of TUNEL positive apoptotic germ cells average in gentamicin, neomycin,streptomycin, ofloxacin and control group were(24.15±10.17), (25.15±9.11), (15.15±11.14), (34.15±8.17), (7.3 ±2.41). The statical analysis was ANOVA method, (P<0.01). Conclusion: Since in our study the rate of TUNEL positive apoptotic germ cells in streptomycin group was significantly less than other treatment groups thus using this drug has fewer risk to consequence infertility in male rats.