Tabriz University of Medical Sciences Medical Journal of Tabriz University of Medical Sciences 2783-2031 35 4 2013 10 06 Metastasis Inhibition through Down Regulation of PlGF in Gastric Adenocarcinoma (AGS) Cells 52 57 EN Ameneh Sharifi Mansour Aminzadeh Bukani Hassan Akrami Mohammad Rahbani Nobar Somayeh Havasi Journal Article 2013 03 17 2013 05 06 Background and Objectives: Placental Growth Factor (PlGF) is a member of Vascular Endothelial Growth Factors (VEGF) family, which can directly act on angiogenesis, tumor cells motility, tumor metastasis, growth and tumor survival. The purpose of this study was to investigate the preventive effect of siRNA on PlGF gene in gastric cancer cells. Materials and Methods: In this experimental study, gastric adenocarcinoma cells (AGS) with 40 picomol concentration of small interfering RNA (siRNA) related to PlGF gene and cellular migration test was carried out, using wound healing model. Results: PlGF gene 24 and 48 hours after of treatment, expression inhibition showed that migration of AGS cells was prevented compared with control condition, but in 72 hours’ time interval, treatment samples showed migration reduction more than control sample. Conclusion: According to these results, reduction of PlGF gene expression may prevents AGS cells migration and metastasis.