Tabriz University of Medical Sciences Medical Journal of Tabriz University of Medical Sciences 2783-2031 45 5 2023 12 06 trans-Chalcone protects male rats against high-fat emulsion-induced pancreatic injury by inhibiting TNF-α and MCP-1 expression 402 410 10.34172/mj.2023.042 EN Elham Karimi-Sales https://orcid.org/0000-0003-1644-5058 Mohammad Reza Alipour https://orcid.org/0000-0002-6484-4267 Journal Article 10.34172/mj.2023.042 2023 03 11 2023 05 10 Background. High-fat diet (HFD) intake is linked to ectopic fat deposition in the pancreas. It also causes pancreatic inflammatory lesions. trans-Chalcone is a simple chalcone with protective effects against HFD-induced metabolic disorders. This study, for the first time, explored the possible effects of this chalcone on high-fat emulsion-induced pancreatic abnormalities in rats. Methods. Twenty-one male rats were randomly assigned into three groups: control (received 10% tween 80); HFD (received high-fat emulsion + 10% tween 80); and HFD + chalcone (received high-fat emulsion + trans-chalcone). Real-time PCR was used to assess pancreatic mRNA expression levels of monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor α (TNF-α) in the pancreas of all rats. Results. High-fat emulsion increased the mRNA expression levels of inflammatory biomarkers, including TNF-α and MCP-1 in the pancreas of rats, and treatment with trans-chalcone prevented these high-fat emulsion-induced changes. Conclusion. trans-Chalcone can protect the pancreas of male rats against HFD-induced abnormalities through its anti-inflammatory effects. Practical Implications. It seems that consumption of HFD up-regulates the production of inflammatory mediators in the pancreas. On the other hand, trans-chalcone can ameliorate HFD-related pancreatic inflammation.